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HPLC Pumps – A deeper look into their types

High performance liquid chromatography, now-a-days, is widely used in biochemistry and analytical chemistry laboratories. The ultimate objective HPLC analysis is to identify, separate and quantify compounds in a sample. HPLC Product comprises of various components, each having its own crucial role to play. HPLC Pump is considered to be one of the most important of such components. Without a pump, the chemical analysis cannot be carried out. Why? Well, because this HPLC part provides the required pressure that drives the sample through the column.

HPLC Accessories such as the pump come in different sizes and types. However a powerful pump has the capacity to produce a pressure up to 6,000 psi, or pounds per square inch, which is applied after the sample is injected. What happens is that it allows the sample to pass through the column more quickly and efficiently than if it were to drip through using only the force of gravity. Before buying HPLC Tools and specifically pump, you should look into various parameters such as flow rate, pressure generation, suitability to different solvents, ease of use etc. Discussed below are some main types of HPLC pumps available in the market today –

  1. Reciprocating Pump – It is one of the most popular pumps used in HPLC. The mobile phase is pushed back & forth action of piston action present about a cylindrical chamber. It is highly sought after because of its constant flow rate, low space occupancy, excellent pressure generation and it is useful for both isocratic & gradient runs.
  2. Pneumatic Pump – One of the simplest of pumps, the Pneumatic Pump as the name goes uses gas to pressurize the mobile phase that is present in a collapsible solvent container. These are considered to be some of the most cost-effective of all HPLC accessories.
  3. Syringe Type HPLC Pump – These pumps generally consist of a cylinder that holds the mobile phase which is expelled by a piston. Now, the piston is advanced by a motor connected through worm gears, to provide smooth pulse less flow. These types of HPLC pumps have their own advantages. Their pressure capability is usually quite high (up to 78,000 psi) and maintenance is infrequent since there are no fluctuating check valves; gears are simple and strong.

It is recommended to purchase HPLC accessories such as the pumps from a renowned & established supplier. The benefits you can get are immense. First, the products offered by such suppliers are best in quality & appropriately priced as well. Next, you can get continuous technical support in one or the other way from professional HPLC spare parts suppliers. So, consult an experienced HPLC supplier, to get the best accessories for your HPLC product.

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The importance HPLC

HPLC is one of the several avatars of Chromatography technique that is being profusely utilised in the laboratories all over the world. Conventionally, this technique is used to separate molecules of comparable mass and run time. Comprising of a mobile medium, a static medium and a chromatogram, this technique operates on the principle of capillary action. The heavier the molecule, the slower it climbs up the static medium. In HPLC, the static medium is invariably a silicone porous layer. HPLC product parts such as HPLC auto samplers, HPLC lamps and HPLC detectors hold a crucial place in HPLC instruments.

The HPLC technique finds its applications mostly in the biological labs around the world. The most widespread application is in isolation peptides and bigger molecules. It is also used to sequence peptides and amino acid molecules. These big biological molecules are less volatile, heavy and take a longer time to climb up the column. It is performed under constant room temperature. This is done basically to keep the molecules from changing their core configurations and getting dysfunctional.

Another utility that this technique finds is in the petroleum industry, although in a limited extent. Heavy petrochemical molecules get separated in the column and can be isolated with precise accuracy. Liquid chromatography has made science obliged in the fact that many a substances could earlier not be separated with accuracy, which meant, impurities stayed behind, hampering the use biological reagents in other experiments and studies.

It is no more the same of course! HPLC has bagged a prime position in laboratories and study houses. HPLC accessories and HPLC kits parts are manufactured and shipped by many laboratory equipment manufacturers and are available in varying precisions and sizes. The better the products and parts, the more accurate the results of HPLC are! It is better to go for the better ones without thinking about the cost. On a long term basis, it proves to be more cost efficient.

Installing a liquid chromatography chamber is nothing that could take a lot on the pocket or the brain. But once installed in a proper manner, under the right conditions, a well devised HPLC apparatus can give stunning results with extremely high accuracy. Liquid chromatography has other sister lanes also, but High performance liquid chromatography is one of the most efficient techniques to isolate and separate molecules. There are ultra high performance liquid chromatography instruments which can withstand high temperatures too.

CTSHPLC is one of the leading online suppliers of HPLC product parts, HPLC accessories and HPLC kits parts of different manufactures including Waters, Beckman, Agilent, Perkin Elmer, Jasco, Gilson, Spectra, Hitachi and many more. We offer high quality HPLC parts including HPLC detectors, HPLC tools, HPLC lamps and HPLC autosamplers at absolutely affordable prices.

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An overview of the Liquid Chromatography Market

Liquid Chromatography (LC) has indeed proved to be a boon for life sciences industry and in particular pharmaceuticals. LC has the ability to segregate a wide variety of chemical mixtures when compared to other chromatography techniques. The market for LC around the world is growing at a healthy rate. Let’s understand what this market and LC process is all about.

What do we mean by Liquid Chromatography?

Liquid chromatography is a separation technique wherein a small amount of liquid sample is injected into a tube that is packed with porous particles (which is often called as the stationary phase). Next, the individual components of the sample are transported along the packed tube (column) by a liquid moved by gravity. Now the components are separated from one another by the column packing that includes different chemical and/or physical interactions between their molecules and the packing particles.

Lastly, the segregated components are collected at the exit of this column and identified by an external measurement technique, such as a spectrophotometer that measures the intensity of the color, or by another device that can measure their amount. Liquid Chromatography is now come to known as Flash Chromatography. HPLC (High Performance/Pressure Liquid Chromatography) is considered to be the best LC technique because of the higher efficiency offered in separating wide range of chemical blends.

Recent Trends and Future of the Liquid Chromatography Market

With introduction of latest technology, LC Market, too, has witnessed ground breaking solutions in the form of introduction of new systems such as fast LC and Ultra HPLC. Organizations are coming together to develop and enhance the throughput and resolution by using smaller particle size columns. It is due to this that the UHPLC systems are introduced which has the remarkable capability to run at extremely high temperatures. The demand for UHPLC instruments is also on the rise.

Next, organizations are now introducing customized systems that are meant for different industries. For instance, HPLC is now used by clinical research organizations for genetic analysis, veterinary sciences and even toxicology. However, with introduction of improved systems often pose new challenges too. Companies are merging to improve their products and services.  Another trend is that the providers of LC equipments are joining hands with software companies for chemoinformatics.

Looking at the activities going on in the LC Market, it is expected that the market will grow to approx $4.7 billion by next year. Out of this US itself will have a share of $1 billion. As the pressure on pharmaceutical industries increases substantially to introduce new drugs in the market, the market for LC instruments and equipments will see a steady growth. The future seems to be bright for HPLC parts makers. Countries such as Japan, India and Puerto Rico will also see manufactures coming and setting up production facilities due to the low cost advantage.

We offers some of the best HPLC equipments and instruments. The Company excels in offering Hitachi HPLC, Hitachi HPLC replacement parts, and even Shimadzu HPLC. If you want to get the best quality HPLC instruments at cost effective rates, then Hitachi HPLC should be there on your list.

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Overview about the important parts of HPLC instruments

High Performance Liquid Chromatography instruments have emerged as one of the most useful tools for industries such as life sciences, pharmaceuticals, petrochemicals, pesticides and lot more. HPLC devices and systems are considered to be a brilliant invention that has helped mankind get better products and services. Using HPLC systems, researchers and scientists can easily segregate molecules from mixtures which would otherwise have been impossible. HPLC systems help separate molecules in the most accurate manner than Gas chromatography devices. Let us understand about the main parts which comprises of a full-fledged HPLC system.

The main function of the HPLC Pump is to force a liquid (which is also known as the mobile phase) via a liquid chromatograph at a specific flow rate, expressed in milliliters per min (mL/min). The solvent is passed through the column using high pressure. Pumps are a vital part of HPLC systems as these help maintain flow rates and high pressure. The flow rate, in addition to how a molecule reacts with the beads in the column, determines how fast a particular chemical will migrate through the column. The migration time is used to identify individual chemicals.

The injector does the task of introducing the liquid sample into the flow stream of the mobile phase. It is mainly used to place a sample into the solvent stream flowing through the column. The Injector is specifically designed to place a liquid sample into the solvent stream with as little perturbation as possible.

Column is considered to be the most important part of the chromatograph which separates the sample components of interest using various physical and chemical parameters. It comprises of a metal housing in a tube shape which is packed with tiny beads that have an affinity for the chemicals that are being analyzed. The chemicals interact with the beads as soon as they flow through the column in a solvent. The minute molecular differences in similar compounds cause them to migrate through the column at different velocities, and separate them according to migration time through the column. The column also has a filter which lets the solvent to flow through but keeps the beads from leaving the column.

It does the work of detecting individual molecules that come out from the column. There are different varieties of detectors that are use for deciding when the chemical should exit the column (the main part of the HPLC system). For instance, the optical HPLC detectors use a beam of light to determine when a particular chemical passes out of the column. When the solvent exits the column, it gets passed through the detector’s light path. Next, there are Ultraviolet and visible light detectors which operate by recording the absorption of light caused by the chemical of interest. Now, the change in absorption caused by the chemical indicates the presence of the chemical. Other detectors that are available are fluorescence and electrochemical detectors.

Data System
This is a computer that helps controls all the modules of the HPLC instrument, takes the signal from the detector and uses it to determine the time of  elution (retention time) of the sample components (qualitative analysis) and the amount of sample (quantitative analysis).

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A comprehensive guide to troubleshooting HPLC systems

High Performance Liquid Chromatography systems better known as HPLC devices are commonly used by life sciences companies to segregate molecules from mixtures for their research and development purposes. The HPLC market around the world is witnessing a steady growth rate, thanks to many advantages offered by HPLC components and HPLC spare parts. HPLC systems are complex in nature. There may arise times when different parts of your HPLC systems encounter technical problems. So, what should be done in such a situation? Well, this article acts as your guide for troubleshooting your HPLC devices.

First of all you need to adopt a logical approach towards resolving issues with HPLC systems. You need to isolate, identify and correct the problems faced by the parts of an HPLC device. One needs to check whether there are any leakage issues with the HPLC system. Most of the devices come equipped with a leakage detector that automatically shuts down the system in case of leaks and moisture. Next, you need to flush the HPLC device and do the replacement for transfer lines. It may happen that the HPLC system might encounter a technical issue due to bacterial growth. The solvents used in your HPLC device don’t have an expiry date but in case the system has not been used for a long period of time, it might lead to bacterial growth. This affects the column in your HPLC device.

For inspecting the column of your HPLC system, you need to monitor the pressure in it for minimum five minutes. For the correct functioning of the column the pressure needs to be stable. The column is an integral part of the HPLC system as the separation of the molecules happens in it. If you find that the column has problems, you need to immediately flush it using hexane and isopropanol. Here you need to remember that the column again needs to be re-flushed with isopropanol before you re-attach it to the hplc product. If all this does not solve the issue, considering replacing the column.

Further, it is important to check the lens of the detector in your HPLC system every month. Regular maintenance and check helps solve many technical problems even before they arise. Also, if you feel that certain components such as piston, check valve, hplc pumps are not functioning in the desired manner, consult your equipment manufacturer, and consider replacing the HPLC components in case of major defects and functional flaws.

Use Hitachi HPLC systems and Hitachi HPLC parts for replacing your existing HPLC components. You can also go for Agilent HPLC parts. Using these HPLC spare parts will help function your HPLC device in the most seamless manner. The HPLC replacement parts offered by these manufactures are excellent in terms of performance, quality and pricing.