HPLC is an analytical tool that quantifies, identifies and separates components in a sample. It is very commonly used system in analytical chemistry and biochemistry fields. The system generally carries the sample using a solvent or mixture of solvents to the stationary phase, where the actual separation of compounds occurs. HPLC analysis is used in routine and non-routine analysis. The HPLC test can be run using parameters established in published literature or methods can also be developed in-house. The HPLC course includes various HPLC Tools such as tubing, syringes, ferrules etc. Students in beginner HPLC programs are provided with an overview of chromatography HPLC Accessories. Ultrasonic cleaning equipment and ultrasonic cleaning HPLC Accessories solve a broad range of analytical sample preparation and degreasing challenges in the industrial and pharmaceutical laboratory.
There are four main systems in a HPLC instrument which helps it to operate in a defined manner. The names of four parts in a HPLC Instrument aremobile phase reservoirs, detector; column and injector system. There are three more other parts which are used in the functioning of the four main parts and they are the recorder, waste and the pump.HPLC Pumpis the most important component and helps the instrument to transfer the mobile phase of the material through the column by providing a high pressure that in turn generates a pressure difference.
A digital liquid HPLC Flow meter is one of the convenient and accurate HPLC Tools used to calibrate HPLC pump flow rates. This tool is also equipped with cleaning HPLC Kits.HPLC Lamps emit an almost continuous spectrum of light ranging from the main UV wavelengths of 160 – 400 nm to the visible spectral range.There is a range of various HPLC Lamps available in the market depending upon its type.
The HPLC Parts are as follows:
- Mobile phase reservoirs: This is the solvent or usually a mixture of solvents used to transport the samples through the whole system and the solvents have to be miscible in the mixture. If not, then the immiscible solvents will cause pressure build-up in the HPLC system.
- Injector Port or HPLC Auto sampler: As the name suggests, this component is used for introducing the sample. The procedure for injecting the samples in to the HPLC auto sampler is manual. In other words, it means that the user has to inject them into the injector using HPLC syringes.Various samples are injected manually into the system by the user and in the end; the appropriate samples are selected by the machine automatically depending on the conditions.
- HPLC Detector: This unit detects the separated compounds in the sample. There are various HPLC Detectors available which use different mode of detection such as refractive index, mass spectroscopy, fluorescence and ultra-violet.
There are various advantages of HPLC and they are high resolution, sensitivity, automation and accuracy. Apart from its advantages, HPLC tool has certain disadvantages which include its high cost, low sensitivity for some compounds and sometimes the process is complex when the irreversibly absorbed compounds are not detected.